Quantitative of progesterone using isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry
Ming-Hui Yang,
Han-Ping You,
Hsin-Yi Wu,
Yi-Ming Arthur Chen,
Ying-Fong Huang,
Yu-Chang Tyan
Affiliations
Ming-Hui Yang
National Mosquito-Borne Diseases Control Research Center, National Health Research Institutes, Miaoli, Taiwan; Clinical Pharmacogenomics and Pharmacoproteomics, College of Pharmacy, Taipei Medical University, Taipei, Taiwan
Han-Ping You
Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan
Hsin-Yi Wu
Instrumentation Center, National Taiwan University, Taipei, Taiwan
Yi-Ming Arthur Chen
Clinical Pharmacogenomics and Pharmacoproteomics, College of Pharmacy, Taipei Medical University, Taipei, Taiwan
Ying-Fong Huang
Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Yu-Chang Tyan
Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan; Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Center for Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan; Corresponding author at: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
A quantification assay based on isotope dilution mass spectrometry to determine the concentration of progesterone in human serum was reported. Incorporated with 13C3-progesterone, serum samples were subjected to progesterone extraction and clean-up by C4 solid-phase-extraction columns and hexane-based liquid/liquid extraction, respectively. The cleaned-up serum samples were then subjected to MALDI-TOF mass spectrometry for the quantification of progesterone. In the study, the recovered progesterone concentration determined by the assay showed good robustness and constancy in comparison to conventional radioimmunologic assay. We concluded that the 13C3-progesterone-based quantification assay is a robust method for the measurement of serum progesterone.Advantages of this technique includes:• This study describes a MALDI-TOF/MS method for the determination of serum progesterone.• The technique is simple and easy to apply on MALDI-TOF/MS for serum progesterone analysis.• The correlation coefficient between MALDI-TOF MS and RIA was 0.981 for serum progesterone.
