Urology Journal (Dec 2008)

Mutations of RAS Gene Family in Specimens of Bladder Cancer

  • Hossein Afshin Alavi,
  • Ali Ahmadi,
  • Amirreza Abedi,
  • Gholamreza Pourmand,
  • Mohammad Taghi Akbari,
  • Abed-Ali Ziaee,
  • Parisa Mousavi-Shafaei,
  • Navaz Karimianpour

Journal volume & issue
Vol. 5, no. 4
pp. 237 – 242

Abstract

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<p class="MsoNormal" style="margin: 0cm 0cm 0pt; direction: ltr; unicode-bidi: embed; text-align: left;"><span style="font-size: small;"><span style="font-family: Times New Roman;"><strong><span style="color: #000000;">Introduction: </span></strong><span style="color: #000000;">Studies have shown different types of <em>RAS</em> mutations in human bladder tumors with a wide range of mutation frequencies in different patient populations. This study aimed to assess the frequency of specific-point mutations in the <em>RAS</em> gene family of a group of Iranian patients with bladder cancer.<strong></strong></span></span></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; direction: ltr; unicode-bidi: embed; text-align: left;"><span style="font-size: small;"><span style="font-family: Times New Roman;"><strong><span style="color: #000000;">Materials and Methods: </span></strong><span style="color: #000000;">We examined the tumor specimens of 35 consecutive patients with transitional cell carcinoma. The<strong> </strong>DNA samples were evaluated for the occurrence of <em>HRAS</em>, <em>KRAS</em>, and <em>NRAS</em> activation using a polymerase chain reaction-restriction fragment length polymorphism technique. </span></span></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; direction: ltr; unicode-bidi: embed; text-align: left;"><span style="font-size: small;"><span style="font-family: Times New Roman;"><strong><span style="color: #000000;">Results:</span></strong><span style="color: #000000;"> None of the patients had mutations in the <em>RAS</em> gene family “hot spots” including codons 12, 13, and 61. <strong></strong></span></span></span></p><strong><span style="font-size: 12pt; color: #000000;">Conclusion: </span></strong><span style="font-size: 12pt; color: #000000;">We failed to find <em>RAS </em>mutations in our bladder tumor samples. These observations may reflect the involvement of different etiological factors in the induction of bladder tumor of which <em>RAS</em> mutation might not be present in all populations. </span>