Bio-Protocol (Sep 2016)

Isolation of Joint-infiltrating Cells

  • Aoi Akitsu,
  • Yoichiro Iwakura

DOI
https://doi.org/10.21769/bioprotoc.1911
Journal volume & issue
Vol. 6, no. 17

Abstract

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Infiltration of leukocytes into joints is one of the main features of autoimmune inflammatory arthritis. Here, we describe the protocol for isolation of joint-infiltrating cells in mice. This protocol is useful to analyze cell surface antigens and intracellular cytokines by flow cytometry.