THE EFFECT OF SANGUIRITRINUM ON THE PATHOGENICITY FACTORS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

Abstract

The relevance of the problem. The frequency of growth of staphylococcal infections in human pathology and the decrease in the effectiveness of their treatment, due to the formation of multidrug resistance, has aroused interest in the study of the anti-staphylococcal activity of Sanguiritrinum and its effect on biological properties, including certain pathogenic factors. The aim of the research was to study the effect of Sanguiritrinum on the pathogenicity factors of the archival strain and clinical isolate of methicillin-resistant S. aureus. Materials and methods. The objects of the study were the reference strain of Staphylococcus aureus ATCC 25923 from the Museum of Living Cultures of Microorganisms of the Department of Microbiology, Virology and Immunology, Poltava State Medical University, and clinical isolate of S. aureus isolated from patients of the surgical department and identified by standard methods. The resistance of this isolate to methicillin was determined using the disk-diffusion method, after which it was concluded that the strain of Staphylococcus aureus belongs to MRSA. In order to assess the antimicrobial activity of the phytopreparation Sanguiritrinum against the studied clinical and archival strains, we used the quantitative method of double serial dilutions in a liquid medium and qualitative disk-diffusion method (Kirby­–Bauer) according to standard methods, in compliance with the Order of the Ministry of Public Health of Ukraine No.167 as of 05.04.2007 on the statement of methodical instructions "Determination of sensitivity of microorganisms to antibacterial drugs". Studies of the effect of Sanguiritrinum on the pathogenicity of S. aureus were performed by assessing the plasma-coagulating, lecithovitelase and hemolytic activity of microorganisms in the presence of plant extract according to conventional methods. Conclusions. The obtained data substantiate the prospects of using Sanguiritrinum in the prevention and treatment of purulent and inflammatory diseases of staphylococcal etiology, including infections caused by methicillin-resistant strains of S. aureus. The phytopreparation Sanguiritrinum has a strong bacteriostatic effect on the reference strain and on the MRSA clinical isolate of S. aureus. Naturally, the clinical isolate of S. aureus with methicillin resistance was characterized by lower sensitivity to the action of Sanguiritrinum, as compared to the sensitivity of the archival strain, because the bactericidal concentration of the drug was 2 times higher. Sanguiritrinum at a concentration of 0.06 μg/ml resulted in complete loss of pathogenicity factors (plasma coagulation, lecithovitelase and hemolytic activity) of the reference strain and the MRSA clinical isolate of S. aureus. At a phytopreparation concentration of 0.03 μg/ml, the microorganisms lost only plasma coagulation ability.

Keywords

• staphylococcus aureus, pathogenicity factors, methicillin-resistant strain, sanguiritrinum, plasma coagulation, lecithovitelase and hemolytic activity