EFFECT OF LEPTIN ON OVOGENESIS DURING DEVELOPMENT IN FEMALE WISTAR RAT

Ghouri Imane; Hanane Bouchefa; Amina Boubekri; Amel Ghozlani; Mohamed Bachir Pacha; Rachid Kaidi; Fatima Hadj-Bekkouch

doi:10.15835/agrisp.v107i3-4.13145

Agricultura (Dec 2018)

EFFECT OF LEPTIN ON OVOGENESIS DURING DEVELOPMENT IN FEMALE WISTAR RAT

Ghouri Imane,
Hanane Bouchefa,
Amina Boubekri,
Amel Ghozlani,
Mohamed Bachir Pacha,
Rachid Kaidi,
Fatima Hadj-Bekkouch

Affiliations

Ghouri Imane: Institute of Veterinary Sciences, University of Blida -1, Route de Soumâa, BP 270, 09 000, Blida; Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Hanane Bouchefa: Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Amina Boubekri: Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Amel Ghozlani: Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Mohamed Bachir Pacha: Institute of Veterinary Sciences, University of Blida -1, Route de Soumâa, BP 270, 09 000, Blida
Rachid Kaidi: Institute of Veterinary Sciences, University of Blida -1, Route de Soumâa, BP 270, 09 000, Blida
Fatima Hadj-Bekkouch: Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers

DOI: https://doi.org/10.15835/agrisp.v107i3-4.13145
Journal volume & issue: Vol. 107, no. 3-4
pp. 188 – 195

Abstract

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The present study aims to investigate the effect of leptin treatment on ovarian weight and ovarian follicle enumeration during development in female Wistar rats. The experiment is carried out on 30 and 60-day-old females. Leptin diluted in 9‰ NaCl is injected daily for 5 days intraperitoneally at a dose of 8 μg / 100 g of body weight to the experimental females. The same volume of the NaCl solution is injected into the controls. Animals are sacrificed 24 hours after the last injection. Ovaries are removed and weighed. The right ovaries are fixed in 10% formaldehyde for histological study. The results reveal a non-significant increase in absolute and relative mean weights of the right and left ovaries in leptin-treated groups compared to controls in both immature and adult rats. The histological study reveals that in 30-day-old females, the number of atretic follicles is most important compared to normal follicles after leptin-treatment, which suggests that leptin appears to stimulate cellular apoptosis at immature age. These results allow us to conclude that leptin seems to influence ovarian weight and follicular atresia.

leptin, ovarian weight, enumeration, follicle, atresia

Published in Agricultura

ISSN: 1221-5317 (Print)
Publisher: AcademicPres
Country of publisher: Romania
LCC subjects: Agriculture: Agriculture (General); Agriculture: Plant culture
Website: http://journals.usamvcluj.ro/index.php/agricultura/index

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