Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Roubina Tatavosian; Huy Nguyen Duc; Thao Ngoc Huynh; Dong Fang; Benjamin Schmitt; Xiaodong Shi; Yiming Deng; Christopher Phiel; Tingting Yao; Zhiguo Zhang; Haobin Wang; Xiaojun Ren

doi:10.1038/s41467-018-04455-7

Nature Communications (May 2018)

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Roubina Tatavosian,
Huy Nguyen Duc,
Thao Ngoc Huynh,
Dong Fang,
Benjamin Schmitt,
Xiaodong Shi,
Yiming Deng,
Christopher Phiel,
Tingting Yao,
Zhiguo Zhang,
Haobin Wang,
Xiaojun Ren

Affiliations

Roubina Tatavosian: Department of Chemistry, University of Colorado Denver
Huy Nguyen Duc: Department of Chemistry, University of Colorado Denver
Thao Ngoc Huynh: Department of Chemistry, University of Colorado Denver
Dong Fang: Institute for Cancer Genetics, Columbia University
Benjamin Schmitt: Department of Biochemistry and Molecular Biology, Colorado State University
Xiaodong Shi: Department of Electrical and Computer Engineering, Michigan State University
Yiming Deng: Department of Electrical and Computer Engineering, Michigan State University
Christopher Phiel: Department of Integrative Biology, University of Colorado Denver
Tingting Yao: Department of Biochemistry and Molecular Biology, Colorado State University
Zhiguo Zhang: Institute for Cancer Genetics, Columbia University
Haobin Wang: Department of Chemistry, University of Colorado Denver
Xiaojun Ren: Department of Chemistry, University of Colorado Denver

DOI: https://doi.org/10.1038/s41467-018-04455-7
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 16

Abstract

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Diffuse intrinsic pontine gliomas exhibit a characteristic mutation of lysine 27 to methionine (K27M) in genes encoding histone H3.3. Here the authors show that the H3.3K27M mutation imposes a specific pattern of H3.3K27 methylation by altering the target search dynamics of PcG proteins.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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