Nature Communications (May 2021)

Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex

  • Joy Y. Wang,
  • Christopher M. Hoel,
  • Basem Al-Shayeb,
  • Jillian F. Banfield,
  • Stephen G. Brohawn,
  • Jennifer A. Doudna

DOI
https://doi.org/10.1038/s41467-021-22900-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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In some systems, a single protein comprising reverse transcriptase (RT), integrase and maturase enables concerted sequence integration and crRNA production. Here, analyses including the structure of a Cas6-RT-Cas1—Cas2 complex suggest coordination between all three active sites and capacity to acquire CRISPR sequences from RNA and DNA substrates.