Molecules (Oct 2022)

Portable Colorimetric Hydrogel Test Kits and On-Mobile Digital Image Colorimetry for On-Site Determination of Nutrients in Water

  • Worawit Wongniramaikul,
  • Bussakorn Kleangklao,
  • Chanita Boonkanon,
  • Tarawee Taweekarn,
  • Kharittha Phatthanawiwat,
  • Wilasinee Sriprom,
  • Wadcharawadee Limsakul,
  • Wanchitra Towanlong,
  • Danai Tipmanee,
  • Aree Choodum

DOI
https://doi.org/10.3390/molecules27217287
Journal volume & issue
Vol. 27, no. 21
p. 7287

Abstract

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Portable colorimetric hydrogel test kits are newly developed for the on-site detection of nitrite, nitrate, and phosphate in water. Griess-doped hydrogel was prepared at the bottom of a 1.5 mL plastic tube for nitrite detection, a nitrate reduction film based on zinc powder was placed on the inner lid of a second 1.5 mL plastic tube for use in conjunction with the Griess-doped hydrogel for nitrate detection, and a molybdenum blue-based reagent was entrapped within a poly(vinyl alcohol) hydrogel matrix placed at the bottom of a third 1.5 mL plastic tube to detect phosphate. These test kits are usable with on-mobile digital image colorimetry (DIC) for the on-site determination of nutrients with good analytical performance. The detection limits were 0.02, 0.04, and 0.14 mg L−1 for nitrite, nitrate, and phosphate, respectively, with good accuracy (−1, undetectable to 2.98 mg L−1, and undetectable to 0.52 mg L−1, respectively.

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