Portable Colorimetric Hydrogel Test Kits and On-Mobile Digital Image Colorimetry for On-Site Determination of Nutrients in Water
Worawit Wongniramaikul,
Bussakorn Kleangklao,
Chanita Boonkanon,
Tarawee Taweekarn,
Kharittha Phatthanawiwat,
Wilasinee Sriprom,
Wadcharawadee Limsakul,
Wanchitra Towanlong,
Danai Tipmanee,
Aree Choodum
Affiliations
Worawit Wongniramaikul
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Bussakorn Kleangklao
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Chanita Boonkanon
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Tarawee Taweekarn
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Kharittha Phatthanawiwat
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Wilasinee Sriprom
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Wadcharawadee Limsakul
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Wanchitra Towanlong
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Danai Tipmanee
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Aree Choodum
Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand
Portable colorimetric hydrogel test kits are newly developed for the on-site detection of nitrite, nitrate, and phosphate in water. Griess-doped hydrogel was prepared at the bottom of a 1.5 mL plastic tube for nitrite detection, a nitrate reduction film based on zinc powder was placed on the inner lid of a second 1.5 mL plastic tube for use in conjunction with the Griess-doped hydrogel for nitrate detection, and a molybdenum blue-based reagent was entrapped within a poly(vinyl alcohol) hydrogel matrix placed at the bottom of a third 1.5 mL plastic tube to detect phosphate. These test kits are usable with on-mobile digital image colorimetry (DIC) for the on-site determination of nutrients with good analytical performance. The detection limits were 0.02, 0.04, and 0.14 mg L−1 for nitrite, nitrate, and phosphate, respectively, with good accuracy (−1, undetectable to 2.98 mg L−1, and undetectable to 0.52 mg L−1, respectively.