Development of a monoclonal antibody to detect αs1-casein in the milk of healthy and mastitis-affected goats

Abstract

This study aimed to evaluate the expression level of caseins during mastitis of goats. Whole goat caseins were used as primary antigens for mouse immunization. A monoclonal antibody (mAb) named 5B with high specificity to goat αs1-casein was developed. Further results showed that mAb 5B can successfully be applied to western blot and ELISA. In addition, immunofluorescence analysis using this mAb showed increased milk αs1-casein level in mastitis-affected goats. In conclusion, this study has established an effective tool to evaluate the expression level of αs1-casein during mastitis development.

Keywords

• αs1-casein
• monoclonal antibody
• elisa
• mastitis
• immunofluorescence
• goat