Application of Phospholipase C from Thielavia terrestris in the Enzymatic Degumming Process of Crude Corn Oil

Abstract

The enzymatic degumming process of crude corn oil using phospholipase C from Thielavia terrestris was studied.The key factors affecting the enzymatic degumming efficiency of crude corn oil,including enzyme dosage, volume ratio of NaOH to citric acid, reaction temperature and reaction time, were optimized by a single-factor experiment with the content of phosphorus in corn oil as the evaluation index.The results showed that the optimal enzymatic degumming conditions were: enzyme dosage of 5000 U/kg,volume ratio (NaOH to citric acid) of 4, reaction temperature of 35 ℃ and reaction time of 1 h.Under the optimized conditions, the phosphorus content was reduced from 102.7 mg/kg to 24.0 mg/kg, with a hydrolysis rate of 76.6%.

Keywords

• crude corn oil
• phospholipase c
• enzymatic degumming
• phosphorus content
• hydrolysis rate