Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit

Dominique Ray-Gallet; M. Daniel Ricketts; Yukari Sato; Kushol Gupta; Ekaterina Boyarchuk; Toshiya Senda; Ronen Marmorstein; Geneviève Almouzni

doi:10.1038/s41467-018-05581-y

Nature Communications (Aug 2018)

Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit

Dominique Ray-Gallet,
M. Daniel Ricketts,
Yukari Sato,
Kushol Gupta,
Ekaterina Boyarchuk,
Toshiya Senda,
Ronen Marmorstein,
Geneviève Almouzni

Affiliations

Dominique Ray-Gallet: Institut Curie, PSL Research University, CNRS, UMR3664, Equipe Labellisée Ligue contre le Cancer
M. Daniel Ricketts: Department of Biochemistry and Biophysics, Graduate Group in Biochemistry and Molecular Biophysics, Abramson Family Cancer Research Institute, Perelman School of Medicine at the University of Pennsylvania
Yukari Sato: Structural Biology Research Center (SBRC), Institute of Materials Structure Science (IMSS), High Energy Accelerator Research Organization (KEK), 1-1 Oho
Kushol Gupta: Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania
Ekaterina Boyarchuk: Institut Curie, PSL Research University, CNRS, UMR3664, Equipe Labellisée Ligue contre le Cancer
Toshiya Senda: Structural Biology Research Center (SBRC), Institute of Materials Structure Science (IMSS), High Energy Accelerator Research Organization (KEK), 1-1 Oho
Ronen Marmorstein: Department of Biochemistry and Biophysics, Graduate Group in Biochemistry and Molecular Biophysics, Abramson Family Cancer Research Institute, Perelman School of Medicine at the University of Pennsylvania
Geneviève Almouzni: Institut Curie, PSL Research University, CNRS, UMR3664, Equipe Labellisée Ligue contre le Cancer

DOI: https://doi.org/10.1038/s41467-018-05581-y
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 15

Abstract

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The HIRA histone chaperone complex is involved in the deposition of the histone variant H3.3. Here the authors, by using biochemical and crystallographic approaches, report the homotrimerization of the HIRA subunit which is critical for the functional activity of the complex.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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