Alginate-based 3D cancer cell culture for therapeutic response modeling

Farideh Davoudi; Samar Ghorbanpoor; Satoshi Yoda; Xiao Pan; Giovanna Stein Crowther; Xunqin Yin; Ellen Murchie; Aaron N. Hata; Henning Willers; Cyril H. Benes

STAR Protocols (Jun 2021)

Alginate-based 3D cancer cell culture for therapeutic response modeling

Farideh Davoudi,
Samar Ghorbanpoor,
Satoshi Yoda,
Xiao Pan,
Giovanna Stein Crowther,
Xunqin Yin,
Ellen Murchie,
Aaron N. Hata,
Henning Willers,
Cyril H. Benes

Affiliations

Farideh Davoudi: Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA; Corresponding author
Samar Ghorbanpoor: Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA
Satoshi Yoda: Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA
Xiao Pan: Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
Giovanna Stein Crowther: Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA
Xunqin Yin: Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA
Ellen Murchie: Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA
Aaron N. Hata: Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA
Henning Willers: Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
Cyril H. Benes: Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 2
p. 100391

Abstract

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Summary: Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients. Here, we describe a protocol for a facile three-dimensional (3D) culture format for cancer cells. This 3D platform helps overcome the 2D culture limitations. In addition, it allows for longitudinal modeling of responses to cancer therapeutics.For complete details on the use and execution of this protocol, please refer to Lhuissier et al. (2017), Lehmann et al. (2016), Liu et al. (2016), and Duval et al. (2011).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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