We report the first confirmed record of Rhynchonycteris naso in southwestern Ecuador. We observed a colony of about 10 individuals roosting under a bridge of a water reservoir that was surrounded by sugar cane crops and located in Naranjito in the Province of Guayas. This is a heavily disturbed area with small remnants of humid evergreen forest in the lowlands of the Ecuadorian coast. This record constitutes a new locality of Rhychonycteris naso for the Guayas province in a different type of vegetation and habitat from previous records for this species in Ecuador and it represents the southwesternmost record for Ecuador and South America.
