Italian Journal of Animal Science (Jan 2010)

Simplified HPLC-UV method for the determination of α-tocopherol in plasma

  • Alberto Bonomi,
  • Afro Quarantelli,
  • Carla Quarantelli,
  • Federico Righi,
  • Marco Renzi

DOI
https://doi.org/10.4081/ijas.2005.191
Journal volume & issue
Vol. 4, no. 2
pp. 191 – 195

Abstract

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Vitamin E, known for its great nutritional importance, is normally included in animal diets as DL-α-tocopherol acetate.The authors propose a method that makes it possible to determine the concentration of vitamin E in plasma withoutsaponification. This method enable to avoid aggressive treatments on the analyte and complex procedures; it detectsvitamin E only in form of DL-α-tocopherol.Lipoproteins of analysed plasma were denaturised by methanol. Vitamin E was extracted by petroleum ether in presenceof NaCl. The extract was dried by rotavapor at 45 °C, solubilized by methanol and injected in HPLC (C18 column, reversedphase). The quantitative determination was carried out by UV detector settled on 294 nm.Tests of repeatability inter-analysis and intra-analysis gave coefficient of variability (CV%) respectively of 1.64 and2.41%. The mean recovery was 100%.

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