The effect of Quinacrine on MMP1 Gene Expression in MDA-MB 231 and MCF7 cell lines of breast cancer

Abstract

Background: Breast cancer is the most frequent malignancy in women worldwide and occurs when cells in the breast begin to grow out of control. Matrix metalloproteinases (MMP), including MMP1 protein, are important in the progression of various types of cancer. Quinacrine, a derivative of 9-aminoacridine, has been shown to inhibit the growth of several types of cancer cells. In this study, we examined the effect of Quinacrine on MMP1 gene expression in breast cancer cell lines MDA-MB 231 and MCF7. MDA-MB 231 cell line has triple negative breast cancer cells properties. Materials and Methods: Breast cancer cell lines, MDA-MB 231 and MCF7, were treated with 0.5 µM Quinacrine for 3 days. The dose was selected using MTT assay. Expression of MMP1 gene was quantified by Real-time PCR. Significance of observations were statistically checked by t-test using P<0.05 as the level of significance. Results: Quinacrine did not have meaningful effect on MMP1 gene expression on MDA-MB 231 and MCF7 cell lines in 0.5 µM concentration for 72 hours. Conclusion: MMP1 gene is important in metastasis and the present study showed that Quinacrine could not affect the expression of this gene on these breast cancer cell lines.

Keywords

• gene expression
• mcf7
• mda-mb 231
• mmp1