Nature Communications (Apr 2019)

Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes

  • Shayesteh R. Ferdosi,
  • Radwa Ewaisha,
  • Farzaneh Moghadam,
  • Sri Krishna,
  • Jin G. Park,
  • Mo R. Ebrahimkhani,
  • Samira Kiani,
  • Karen S. Anderson

DOI
https://doi.org/10.1038/s41467-019-09693-x
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 10

Abstract

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Possible immunogenicity of the Cas9 protein raises concerns about therapeutic applications. Here the authors identify pre-existing CD8+T-cell immunity in healthy individuals and in response modify Cas9 to remove the immunodominant epitopes.