Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts

Mitchell R. Harancher; Meena Sukhwani; Carlos A. Castro; Michael H. Hsieh; Kyle E. Orwig

Stem Cell Research (Dec 2023)

Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts

Mitchell R. Harancher,
Meena Sukhwani,
Carlos A. Castro,
Michael H. Hsieh,
Kyle E. Orwig

Affiliations

Mitchell R. Harancher: Molecular Genetics and Developmental Biology Graduate Program, University of Pittsburgh School of Medicine, USA; Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USA
Meena Sukhwani: Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USA
Carlos A. Castro: Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USA
Michael H. Hsieh: Children’s National Hospital, Washington, DC, USA
Kyle E. Orwig: Molecular Genetics and Developmental Biology Graduate Program, University of Pittsburgh School of Medicine, USA; Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USA; Corresponding author.

Journal volume & issue: Vol. 73
p. 103257

Abstract

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Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves testicular tissues for SCD patients prior to BMT in anticipation that those tissues can be thawed in the future and matured to produce sperm. Here, we generated and validated two isogenic patient-derived induced pluripotent stem cell (iPSC) lines from testicular biopsy fibroblasts of a 12-year-old SCD patient.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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