Detection of antibodies against Prevotella Intermedia in patients with chronic periodontitis and periodontally healthy individuals

Abstract

Background: Periodontitis, an inflammatory disease of multifactorial etiology, has bacteria playing an essential role in its pathogenesis. Prevotella intermedia plays an important role in disease initiation and progression. Objectives: The objective was to detect immunoglobulin G (IgG) antibodies against P. intermedia in blood of periodontally healthy individuals and patients with chronic periodontitis and compare their levels. Materials and Methodology: A total of 72 subjects were included, 36 subjects in the healthy group and 36 subjects in the chronic periodontitis group. Subgingival plaque sample and blood sample were obtained from each study subject. Samples were processed in the Department of Microbiology and Immunology. P. intermedia were confirmed using the culture method and serum IgG levels were assessed using ELISA (enzyme-linked immunosorbent assay) technique. Comparison between healthy and chronic periodontitis groups was done using independent t-test. Results: IgG levels against P. intermedia were more in the chronic periodontitis group compared to the healthy group, and the difference was statistically significant. Interpretations and Conclusions: Increased levels of IgG antibodies against P. intermedia are associated with periodontal disease. This elevated antibody activity might help to neutralize the effects of the bacterium. IgG antibody level against P. intermedia is a promising indicator in the serological diagnosis of periodontal disease. In chronic periodontitis, the antibody titer in the patient's serum against P. intermedia is raised and could be used as a diagnostic aid.

Keywords

• elisa
• prevotella intermedia
• periodontal disease
• serum immunoglobulin g