Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure

Abstract

Summary: We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal, intramuscular, and intranasal routes, generating single-cell suspensions from the inoculated and target organs for downstream analyses.For complete details on the use and execution of this protocol, please refer to Ndeupen et al. (2021) and (2022).

Keywords

• Health Sciences
• Immunology