Therapeutic potential of probiotic Lactobacillus plantarum AD3 on acetaminophen induced uremia in experimental rats

Abstract

Summary: Background & aims: Chronic kidney disease (CKD) is rapidly heading towards epidemic proportion globally, requires dialysis or kidney transplantation. No generally applicable therapies to slow progression of renal disease are available. The aim of this study was to evaluate Lactobacillus plantarum AD3 as an effective probiotic strain for acetaminophen induced uremic patient. Methods: Plasma uremic profiles, oxidative stress marker, DNA fragmentation assay of kidney tissue and kidney histological studies were investigated on acetaminophen-induced uremic rats (Wister strain albino male). Limited fecal matter analysis and HPLC of intestinal fluid were also performed. Results: In APAP treated rats, plasma urea, creatinine (Cr), glutamate oxaloacetate transaminase (GOT) and malonaldehyde (MDA) level elevated significantly compared to negative control. However, level of plasma urea, creatinine, GOT and MDA in tested rats were significantly lower in comparison to uremic control (p < 0.05). Electrolytes profile of the tested plasma samples were in acceptable range. Glomerular necrosis, DNA damage of uremic rats were also significantly lowered. In contrast, SCFAs have been associated with attenuated kidney damage by protecting against oxidative stress. Conclusion: The urease positive probiotic L. plantarum AD3 strain have the antioxidative and antiuremic efficacy in acetaminophen induced experimental rats. Keywords: Acetaminophen induced uremia, Urease positive probiotic, Lactobacillus plantarum AD3, Rat model