Prevalence and antimicrobial-resistant characterization of Bacillus cereus isolated from ready-to-eat rice products in Eastern China

Jiancai Chen; Junyan Zhang; Li Zhan; Honghu Chen; Zhen Zhang; Cheng Huang; Min Yue; Min Yue; Min Yue; Min Yue

doi:10.3389/fmicb.2022.964823

Frontiers in Microbiology (Jul 2022)

Prevalence and antimicrobial-resistant characterization of Bacillus cereus isolated from ready-to-eat rice products in Eastern China

Jiancai Chen,
Junyan Zhang,
Li Zhan,
Honghu Chen,
Zhen Zhang,
Cheng Huang,
Min Yue,
Min Yue,
Min Yue,
Min Yue

Affiliations

Jiancai Chen: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Junyan Zhang: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Li Zhan: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Honghu Chen: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Zhen Zhang: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Cheng Huang: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Min Yue: Hainan Institute, Zhejiang University, Hangzhou, China
Min Yue: Institute of Preventive Veterinary Sciences and Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China
Min Yue: Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, China
Min Yue: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China

DOI: https://doi.org/10.3389/fmicb.2022.964823
Journal volume & issue: Vol. 13

Abstract

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Bacillus cereus is a major food-borne bacterial pathogen in the world, which can cause diarrhea and emetic syndrome. This study aimed to reveal the quantitative prevalence of B. cereus in ready-to-eat (RTE) rice products in Eastern China and to gain essential information on the characteristics of B. cereus isolates. A total of 91 out of the 1071 samples were positive for B. cereus. The contamination level of B. cereus in 0.5 % of RTE rice product samples outnumbered 103 CFU/g. The number of B. cereus attained 105−106 CFU/g in one sample. The distribution patterns of virulence genes in B. cereus isolates were identified. 84.6% of the B. cereus isolates had at least one enterotoxin or emetic toxin gene. The predominant pattern was XXV. 9.9% of isolates belonged to it and possessed one enterotoxin gene entFM. The occurrence rate of hblACD and nheABC was 36.3% and 47.3%, respectively. Antimicrobial susceptibility tests revealed a high resistance rate toward penicillin, and 23.1% of the isolates were multi-drug resistant. B. cereus isolates were genotyped by using ERIC-PCR. 89 genotypes were determined. The Hunter Gaston Discriminatory Index (HGDI) attained 0.9995. Relationships analysis revealed that Group A B. cereus isolates tended to carry hblA, hblC, hblD, nheA, nheB, and show resistance to penicillin/trimethoprim/sulfamethoxazole. This study was useful for updating the knowledge of the contamination status of B. cereus in RTE rice products in China.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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