Frontiers in Medicine (Oct 2021)
Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis
- Lingling Shen,
- Lingling Shen,
- Lingling Shen,
- Lingling Shen,
- Lan Lan,
- Lan Lan,
- Lan Lan,
- Lan Lan,
- Tingting Zhu,
- Tingting Zhu,
- Tingting Zhu,
- Tingting Zhu,
- Hongjun Chen,
- Hongjun Chen,
- Hongjun Chen,
- Hongjun Chen,
- Haifeng Gu,
- Cuili Wang,
- Cuili Wang,
- Cuili Wang,
- Cuili Wang,
- Ying Chen,
- Ying Chen,
- Ying Chen,
- Ying Chen,
- Minmin Wang,
- Haiyan Tu,
- Philipp Enghard,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang,
- Jianghua Chen,
- Jianghua Chen,
- Jianghua Chen,
- Jianghua Chen
Affiliations
- Lingling Shen
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Lingling Shen
- Key Laboratory of Nephropathy, Hangzhou, China
- Lingling Shen
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Lingling Shen
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, China
- Lan Lan
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Lan Lan
- Key Laboratory of Nephropathy, Hangzhou, China
- Lan Lan
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Lan Lan
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, China
- Tingting Zhu
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Tingting Zhu
- Key Laboratory of Nephropathy, Hangzhou, China
- Tingting Zhu
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Tingting Zhu
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, China
- Hongjun Chen
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Hongjun Chen
- Key Laboratory of Nephropathy, Hangzhou, China
- Hongjun Chen
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Hongjun Chen
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, China
- Haifeng Gu
- Department of Geriatrics, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Cuili Wang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Cuili Wang
- Key Laboratory of Nephropathy, Hangzhou, China
- Cuili Wang
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Cuili Wang
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, China
- Ying Chen
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Ying Chen
- Key Laboratory of Nephropathy, Hangzhou, China
- Ying Chen
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Ying Chen
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, China
- Minmin Wang
- Department of Nephrology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, China
- Haiyan Tu
- Department of Nephrology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
- Philipp Enghard
- Department of Nephrology and Medical Intensive Care, Charité - Universitätsmedizin Berlin, Berlin, Germany
- Hong Jiang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Hong Jiang
- Key Laboratory of Nephropathy, Hangzhou, China
- Hong Jiang
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Hong Jiang
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, China
- Jianghua Chen
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Jianghua Chen
- Key Laboratory of Nephropathy, Hangzhou, China
- Jianghua Chen
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Jianghua Chen
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, China
- DOI
- https://doi.org/10.3389/fmed.2021.762848
- Journal volume & issue
-
Vol. 8
Abstract
Lupus nephritis (LN) is a common and severe organ manifestation of systemic lupus erythematosus (SLE) and is a major cause of SLE related deaths. Early diagnosis is essential to improve the prognosis of patients with LN. To screen the potential biomarkers associated with LN, we downloaded the gene expression profile of GSE99967 from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) was utilized to construct a gene co-expression network and identify gene modules associated with LN. Gene Ontology (GO) analysis was also applied to explore the biological function of genes and identify the key module. Differentially expressed genes (DEGs) were identified and Maximal Clique Centrality (MCC) values were calculated to screen hub genes. Furthermore, we selected promising biomarkers for real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) validation in independent cohorts. Our results indicated that five hub genes, including IFI44, IFIT3, HERC5, RSAD2, and DDX60 play vital roles in the pathogenesis of LN. Importantly, IFI44 may considered as a key biomarker in LN for its diagnostic capabilities, which is also a promising therapeutic target in the future.
Keywords