Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice

Abstract

Summary: Here, we present a protocol to create an in vivo lineage-tracing mouse model for mouse-papillomavirus-type 1 (MmuPV1)-infected cells. We describe the steps to generate and deliver the MmuPV1 lox-Cre-lox plasmid for the infection of mice, followed by skin tissue extraction and processing. We then detail how to use flow cytometry to trace, quantify, and analyze MmuPV1-harboring cells and their progeny. This model is suitable to investigate the early effects of papillomavirus on the target cells.For complete details on the use and execution of this protocol, please refer to Yilmaz et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell Biology
• Flow Cytometry/Mass Cytometry
• Microbiology
• Model Organisms
• Molecular Biology