Transcriptome datasets of neural progenitors and neurons differentiated from induced pluripotent stem cells of healthy donors and Parkinson's disease patients with mutations in the PARK2 gene
Ekaterina Novosadova,
Ksenia Anufrieva,
Elizaveta Kazantseva,
Elena Arsenyeva,
Viya Fedoseyeva,
Ekaterina Stepanenko,
Daniil Poberezhniy,
Sergey Illarioshkin,
Lyudmila Novosadova,
Tatiana Gerasimova,
Valentina Nenasheva,
Igor Grivennikov,
Maria Lagarkova,
Vyacheslav Tarantul
Affiliations
Ekaterina Novosadova
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Ksenia Anufrieva
Federal Research and Clinical Center of Physical Chemical Medicine of the Federal Medical and Biological Agency of the Russian Federation, Moscow, Russia
Elizaveta Kazantseva
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Elena Arsenyeva
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Viya Fedoseyeva
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Ekaterina Stepanenko
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Daniil Poberezhniy
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia; Faculty of Biotechnology and Industrial Ecology, D.I. Mendeleyev University of Chemical Technology of Russia, Moscow, Russia
Sergey Illarioshkin
Research Center of Neurology, Moscow, Russia
Lyudmila Novosadova
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Tatiana Gerasimova
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Valentina Nenasheva
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia; Corresponding author.
Igor Grivennikov
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Maria Lagarkova
Federal Research and Clinical Center of Physical Chemical Medicine of the Federal Medical and Biological Agency of the Russian Federation, Moscow, Russia
Vyacheslav Tarantul
Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, Russia
Parkinson's disease (PD) is a complex systemic disorder caused by neurodegenerative processes in the brain that are mainly characterized by progressive loss of dopaminergic neurons in the substantia nigra. About 10% of PD cases have been linked to specific gene mutations (Zafar and Yaddanapudi, 2022) including the PARK2 gene that encodes a RING domain-containing E3 ubiquitin ligase Parkin. PD-Parkin patients have a younger onset, longer disease duration, and more severe clinical symptoms in comparison to PD patients with unknown causative PD mutations (Zhou et al., 2020). Induced pluripotent stem cells (iPSCs) are considered to be a powerful tool for disease modeling. To evaluate how mutations in PARK2 contribute to PD development, iPSC lines were obtained from three healthy donors and three PD patients with different mutations in the PARK2 gene. iPSC lines were differentiated consequently into neural progenitors (NPs) and then into terminally differentiated neurons (DNs). The data presented in this article were generated on an NextSeq 500 System (Illumina) and include transcriptome profiles for NPs and DNs of healthy donors and PD patients with mutations in the PARK2 gene. Top10 up- and down-regulated differentially expressed genes in NPs and DNs of patients with PD compared to healthy donors were also presented. A comparative transcriptome analysis of neuronal derivatives of healthy donors and PD patients allows to examine the contributions of the PARK2 gene mutations to PD pathogenesis.
