Gut microbiota dysbiosis-related susceptibility to nontuberculous mycobacterial lung disease
Tzu-Lung Lin,
Yen-Liang Kuo,
Juo-Hsin Lai,
Chia-Chen Lu,
Chang-Tsu Yuan,
Chi-Yu Hsu,
Bo-Shiun Yan,
Lawrence Shih-Hsin Wu,
Ting-Shu Wu,
Jann-Yuan Wang,
Chong-Jen Yu,
Hsin-Chih Lai,
Jwu-Ching Shu,
Chin-Chung Shu
Affiliations
Tzu-Lung Lin
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan
Yen-Liang Kuo
Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan
Juo-Hsin Lai
Internal Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
Chia-Chen Lu
REVIVEBIO CO, Taipei city, Taiwan
Chang-Tsu Yuan
Graduate Institute of Clinical Medicine, National Taiwan University, Taipei, Taiwan
Chi-Yu Hsu
Internal Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
Bo-Shiun Yan
Institute of Biochemistry and Molecular Biology, National Taiwan University College of Medicine, Taipei City, Taiwan
Lawrence Shih-Hsin Wu
Graduate Institute of Biomedical Sciences, China Medical University, Taichung City, Taiwan
Ting-Shu Wu
Division of Infectious Diseases, Department of Internal Medicine, Linkou Chang Gung Memorial Hospital, Chang Gung University School of Medicine, Taoyuan, Taiwan
Jann-Yuan Wang
Internal Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
Chong-Jen Yu
Internal Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
Hsin-Chih Lai
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan
Jwu-Ching Shu
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan
Chin-Chung Shu
Internal Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
The role of gut microbiota in host defense against nontuberculous mycobacterial lung disease (NTM-LD) was poorly understood. Here, we showed significant gut microbiota dysbiosis in patients with NTM-LD. Reduced abundance of Prevotella copri was significantly associated with NTM-LD and its disease severity. Compromised TLR2 activation activity in feces and plasma in the NTM-LD patients was highlighted. In the antibiotics-treated mice as a study model, gut microbiota dysbiosis with reduction of TLR2 activation activity in feces, sera, and lung tissue occurred. Transcriptomic analysis demonstrated immunocompromised in lung which were closely associated with increased NTM-LD susceptibility. Oral administration of P. copri or its capsular polysaccharides enhanced TLR2 signaling, restored immune response, and ameliorated NTM-LD susceptibility. Our data highlighted the association of gut microbiota dysbiosis, systematically compromised immunity and NTM-LD development. TLR2 activation by P. copri or its capsular polysaccharides might help prevent NTM-LD.
