Bioactive Compounds and Evaluation of Antioxidant, Cytotoxic and Cytoprotective Effects of Murici Pulp Extracts (<i>Byrsonima crassifolia</i>) Obtained by Supercritical Extraction in HepG2 Cells Treated with H<sub>2</sub>O<sub>2</sub>
Flávia Cristina Seabra Pires,
Joicy Corrêa de Oliveira,
Eduardo Gama Ortiz Menezes,
Ana Paula de Souza e Silva,
Maria Caroline Rodrigues Ferreira,
Leticia Maria Martins Siqueira,
Andryo Orfi Almada-Vilhena,
Julio Cesar Pieczarka,
Cleusa Yoshiko Nagamachi,
Raul Nunes de Carvalho Junior
Affiliations
Flávia Cristina Seabra Pires
LABEX (Extraction Laboratory), LABTECS (Supercritical Technology Laboratory), PPGCTA (PostGraduate Program in Food Science and Technology), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Joicy Corrêa de Oliveira
LABEX (Extraction Laboratory), FEA (College of Food Engineering), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Eduardo Gama Ortiz Menezes
LABEX (Extraction Laboratory), PRODERNA (Postgraduate Program in Natural Resources Engineering in the Amazon), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Ana Paula de Souza e Silva
LABEX (Extraction Laboratory), LABTECS (Supercritical Technology Laboratory), PPGCTA (PostGraduate Program in Food Science and Technology), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Maria Caroline Rodrigues Ferreira
LABEX (Extraction Laboratory), LABTECS (Supercritical Technology Laboratory), PPGCTA (PostGraduate Program in Food Science and Technology), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Leticia Maria Martins Siqueira
LABEX (Extraction Laboratory), PRODERNA (Postgraduate Program in Natural Resources Engineering in the Amazon), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Andryo Orfi Almada-Vilhena
CEABIO (Center for Advanced Studies of the Biodiversity and Cell Culture Laboratory), PCT-Guamá (Guamá Science and Technology Park), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Julio Cesar Pieczarka
CEABIO (Center for Advanced Studies of the Biodiversity and Cell Culture Laboratory), PCT-Guamá (Guamá Science and Technology Park), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Cleusa Yoshiko Nagamachi
CEABIO (Center for Advanced Studies of the Biodiversity and Cell Culture Laboratory), PCT-Guamá (Guamá Science and Technology Park), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
Raul Nunes de Carvalho Junior
LABEX (Extraction Laboratory), LABTECS (Supercritical Technology Laboratory), FEA (College of Food Engineering), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil
The use of clean technologies in the development of bioactive plant extracts has been encouraged, but it is necessary to verify the cytotoxicity and cytoprotection for food and pharmaceutical applications. Therefore, the objective of this work was to obtain the experimental data of the supercritical sequential extraction of murici pulp, to determine the main bioactive compounds obtained and to evaluate the possible cytotoxicity and cytoprotection of the extracts in models of HepG2 cells treated with H2O2. The murici pulp was subjected to sequential extraction with supercritical CO2 and CO2+ethanol, at 343.15 K, and 22, 32, and 49 MPa. Higher extraction yields were obtained at 49 MPa. The oil presented lutein (224.77 µg/g), oleic, palmitic, and linoleic, as the main fatty acids, and POLi (17.63%), POO (15.84%), PPO (13.63%), and LiOO (10.26%), as the main triglycerides. The ethanolic extract presented lutein (242.16 µg/g), phenolic compounds (20.63 mg GAE/g), and flavonoids (0.65 mg QE/g). The ethanolic extract showed greater antioxidant activity (122.61 and 17.14 µmol TE/g) than oil (43.48 and 6.04 µmol TE/g). Both extracts did not show cytotoxicity and only murici oil showed a cytoprotective effect. Despite this, the results qualify both extracts for food/pharmaceutical applications.
