miR-300/FA2H affects gastric cancer cell proliferation and apoptosis
Hong Bo,
Li Jie,
Huang Chunxiao,
Huang Tao,
Zhang Mengpei,
Huang Lijiang
Affiliations
Hong Bo
Department of Gastroenterology, Xiangshan Hospital Affiliated to Wenzhou Medical University, 291 Donggu Road, Dandong Street, Xiangshan County, Ningbo, 315700, People’s Republic of China
Li Jie
Department of Gastroenterology, Xiangshan Hospital Affiliated to Wenzhou Medical University, 291 Donggu Road, Dandong Street, Xiangshan County, Ningbo, 315700, People’s Republic of China
Huang Chunxiao
Department of Gastroenterology, Xiangshan Hospital Affiliated to Wenzhou Medical University, 291 Donggu Road, Dandong Street, Xiangshan County, Ningbo, 315700, People’s Republic of China
Huang Tao
Department of Gastroenterology, Xiangshan Hospital Affiliated to Wenzhou Medical University, 291 Donggu Road, Dandong Street, Xiangshan County, Ningbo, 315700, People’s Republic of China
Zhang Mengpei
Department of Gastroenterology, Xiangshan Hospital Affiliated to Wenzhou Medical University, 291 Donggu Road, Dandong Street, Xiangshan County, Ningbo, 315700, People’s Republic of China
Huang Lijiang
Department of Gastroenterology, Xiangshan Hospital Affiliated to Wenzhou Medical University, 291 Donggu Road, Dandong Street, Xiangshan County, Ningbo, 315700, People’s Republic of China
MicroRNA (miR/miRNA) expression disorders play a crucial role in the development of gastric cancer (GC). Increasing evidence has indicated that miRNAs participate in the process of numerous cancers. Previous research has demonstrated that miR-300 acts as a cancer-promoting factor or tumor suppressor in a number of tumors. However, to the best of our knowledge, the effects of miR-300 on GC cells remain largely unknown. The present study investigated the effects of miR-300 on GC cells and analyzed its molecular mechanism. First, reverse transcription–quantitative polymerase chain reaction showed that miR-300 expression was increased in GC tissues and cell lines, with the highest expression observed in human gastric cancer cell line AGS. Subsequent results indicated that fatty acid 2-hydroxylase (FA2H) was a target of miR-300. FA2H-plasmid inhibited AGS cell proliferation and induced apoptosis. Finally, miR-300 inhibitor reduced cell proliferation and induced apoptosis, whereby these effects were reversed by FA2H-small interfering RNA. Therefore, the data demonstrated that miR-300/FA2H might be a new potential biomarker and therapeutic target for GC treatment.
