Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1

Jiyeon Kweon; An-Hee Jang; Da-eun Kim; Jin Wook Yang; Mijung Yoon; Ha Rim Shin; Jin-Soo Kim; Yongsub Kim

doi:10.1038/s41467-017-01650-w

Nature Communications (Nov 2017)

Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1

Jiyeon Kweon,
An-Hee Jang,
Da-eun Kim,
Jin Wook Yang,
Mijung Yoon,
Ha Rim Shin,
Jin-Soo Kim,
Yongsub Kim

Affiliations

Jiyeon Kweon: Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center
An-Hee Jang: Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center
Da-eun Kim: Department of Chemistry, Seoul National University
Jin Wook Yang: Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center
Mijung Yoon: Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center
Ha Rim Shin: Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center
Jin-Soo Kim: Department of Chemistry, Seoul National University
Yongsub Kim: Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center

DOI: https://doi.org/10.1038/s41467-017-01650-w
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 6

Abstract

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Cas9 and Cpf1 have both been adapted for genome engineering, editing and gene expression regulation. Here the authors design a fusion guide RNA that can interact with both proteins for multiple and orthogonal genome manipulation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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