Известия высших учебных заведений. Поволжский регион: Медицинские науки (Mar 2020)

STUDYING THE ROLE OF GENES POLYMORPHISM OF CYTOKINES AND TOLL-LIKE RECEPTORS IN PYODERMA PATHOGENESIS

  • N. I. Baranova,
  • N. Yu. Alekseeva,
  • N. I. Mikulyak

DOI
https://doi.org/10.21685/2072-3032-2020-1-12
Journal volume & issue
no. 1

Abstract

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Background. At present many authors associate pathogenesis with the defect in genes polymorphism of key cytokines and the genes defect of Toll-like receptors (TLRs) of congenital immune system. The objective of this study was to analyze key cytokines indicators IL-4, IL-6, IL-10, IL-18, FNO-α, TGF-β, IFN-γ, associations of genes polymorphism IL-2, IL-4, IL-17A and TLR2, TLR4, TLR3, TLR9 in pyoderma patients. Materials and methods. Over the study period 98 patients with pyoderma (chronic recurrent furuncles, sycosis) were examined 45 healthy persons (control group) were examined as well. The age was from 18 to 65. The indicators IL-10, IL- 18, TGF-β, IL-4 in supernatants of stimulated cells had been study was determined by enzyme immunoassay. The variants of genes IL-2, IL-4, IL-17A и TLR2, TLR4, TLR3, TLR9 were typified by polymerase chain reaction with the detection of amplification products in the «real time» mode. Results. The increase of indicators IL-10, IL-18, TGF-β, IL-4 in blood serum and the decrease of indicators of induced IFN- γ were identifiti in pyoderma patients comparing to control group. This fact proves that pyoderma patients are in immunodeficiency condition by humoral type with predominated Th2 immune response. The increase of genotype prevalency A/A of polymorphism (G-197A) of gene IL- 17A in pyoderma patients can be related to the impact deficiency of Th17 on neutrophils. It can result in predisposition to skin diseases of infectious genesis. The decrease of genotype A/A and increase of genotype A/G polymorphism (A-753G) of gene TLR-2 in pyoderma patients can result in lack of response into lipopolysaccharids and selective disfunction of protecting reactions against gram-positive bacteria. Conclusions. Predominance of Th2 type of immune response and revealed defects of polymorphism (G-197A) of IL-17A gene and polymorphism) (A-753G of TLR2 gene in patients with pyoderma will contribute to understanding of immunogenetic bases of disease formation for use in clinical practice.

Keywords