Fabrication of Flexible Arrayed Lactate Biosensor Based on Immobilizing LDH-NAD+ on NiO Film Modified by GO and MBs

Jung-Chuan Chou; Siao-Jie Yan; Yi-Hung Liao; Chih-Hsien Lai; You-Xiang Wu; Cian-Yi Wu; Hsiang-Yi Chen; Hong-Yu Huang; Tong-Yu Wu

doi:10.3390/s17071618

Sensors (Jul 2017)

Fabrication of Flexible Arrayed Lactate Biosensor Based on Immobilizing LDH-NAD+ on NiO Film Modified by GO and MBs

Jung-Chuan Chou,
Siao-Jie Yan,
Yi-Hung Liao,
Chih-Hsien Lai,
You-Xiang Wu,
Cian-Yi Wu,
Hsiang-Yi Chen,
Hong-Yu Huang,
Tong-Yu Wu

Affiliations

Jung-Chuan Chou: Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
Siao-Jie Yan: Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
Yi-Hung Liao: Department of Information and Electronic Commerce Management, TransWorld University, Douliu 64063, Taiwan
Chih-Hsien Lai: Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
You-Xiang Wu: Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
Cian-Yi Wu: Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
Hsiang-Yi Chen: Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
Hong-Yu Huang: Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
Tong-Yu Wu: Graduate School of Mechanical Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan

DOI: https://doi.org/10.3390/s17071618
Journal volume & issue: Vol. 17, no. 7
p. 1618

Abstract

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We proposed the flexible arrayed lactate biosensor based on immobilizing l-lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide ( NAD + ) on nickel oxide (NiO) film, and which the average sensitivity could be enhanced by using graphene oxide (GO) and magnetic beads (MBs). By using GO and MBs, it exhibits excellent sensitivity (45.397 mV/mM) with a linearity of 0.992 in a range of 0.2 mM to 3 mM. According to the results of electrochemical impedance spectroscopy (EIS), the electron transfer resistance of LDH- NAD + -MBs/GPTS/GO/NiO film was smaller than those of LDH-NAD+/GPTS/GO/NiO film and LDH- NAD + /GPTS/NiO film, and it presented the outstanding electron transfer ability. After that, the limit of detection, anti-interference effect and bending test were also investigated.

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

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