Frontiers in Veterinary Science (Mar 2023)
RNA sequencing reveals CircRNA expression profiles in chicken embryo fibroblasts infected with velogenic Newcastle disease virus
- Libin Chen,
- Libin Chen,
- Libin Chen,
- Libin Chen,
- Jiayu Ruan,
- Jiayu Ruan,
- Jiayu Ruan,
- Jiayu Ruan,
- Yiyi Chen,
- Yiyi Chen,
- Yiyi Chen,
- Yiyi Chen,
- Wenxuan Deng,
- Wenxuan Deng,
- Wenxuan Deng,
- Wenxuan Deng,
- Jinyu Lai,
- Jinyu Lai,
- Jinyu Lai,
- Jinyu Lai,
- Lei Fan,
- Lei Fan,
- Lei Fan,
- Lei Fan,
- Juncheng Cai,
- Juncheng Cai,
- Juncheng Cai,
- Juncheng Cai,
- Chan Ding,
- Qiuyan Lin,
- Qiuyan Lin,
- Qiuyan Lin,
- Qiuyan Lin,
- Bin Xiang,
- Tao Ren,
- Tao Ren,
- Tao Ren,
- Tao Ren
Affiliations
- Libin Chen
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Libin Chen
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Libin Chen
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Libin Chen
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Jiayu Ruan
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Jiayu Ruan
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Jiayu Ruan
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Jiayu Ruan
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Yiyi Chen
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yiyi Chen
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Yiyi Chen
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Yiyi Chen
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Wenxuan Deng
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Wenxuan Deng
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Wenxuan Deng
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Wenxuan Deng
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Jinyu Lai
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Jinyu Lai
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Jinyu Lai
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Jinyu Lai
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Lei Fan
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Lei Fan
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Lei Fan
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Lei Fan
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Juncheng Cai
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Juncheng Cai
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Juncheng Cai
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Juncheng Cai
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Chan Ding
- Shanghai Veterinary Research Institute (SHVRI), Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China
- Qiuyan Lin
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Qiuyan Lin
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Qiuyan Lin
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Qiuyan Lin
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Bin Xiang
- College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan, China
- Tao Ren
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Tao Ren
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China
- Tao Ren
- National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China
- Tao Ren
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- DOI
- https://doi.org/10.3389/fvets.2023.1167444
- Journal volume & issue
-
Vol. 10
Abstract
IntroductionNewcastle disease virus (NDV) is an important avian pathogen prevalent worldwide; it has an extensive host range and seriously harms the poultry industry. Velogenic NDV strains exhibit high pathogenicity and mortality in chickens. Circular RNAs (circRNAs) are among the most abundant and conserved eukaryotic transcripts. They are part of the innate immunity and antiviral response. However, the relationship between circRNAs and NDV infection is unclear.MethodsIn this study, we used circRNA transcriptome sequencing to analyze the differences in circRNA expression profiles post velogenic NDV infection in chicken embryo fibroblasts (CEFs). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to reveal significant enrichment of differentially expressed (DE) circRNAs. The circRNA- miRNA-mRNA interaction networks were further predicted. Moreover, circ-EZH2 was selected to determine its effect on NDV infection in CEFs.ResultsNDV infection altered circRNA expression profiles in CEFs, and 86 significantly DE circRNAs were identified. GO and KEGG enrichment analyses revealed significant enrichment of DE circRNAs for metabolism-related pathways, such as lysine degradation, glutaminergic synapse, and alanine, aspartic-acid, and glutamic-acid metabolism. The circRNA- miRNA-mRNA interaction networks further demonstrated that CEFs might combat NDV infection by regulating metabolism through circRNA-targeted mRNAs and miRNAs. Furthermore, we verified that circ-EZH2 overexpression and knockdown inhibited and promoted NDV replication, respectively, indicating that circRNAs are involved in NDV replication.ConclusionsThese results demonstrate that CEFs exert antiviral responses by forming circRNAs, offering new insights into the mechanisms underlying NDV-host interactions.
Keywords