A multiplex platform for small RNA sequencing elucidates multifaceted tRNA stress response and translational regulation

Christopher P. Watkins; Wen Zhang; Adam C. Wylder; Christopher D. Katanski; Tao Pan

doi:10.1038/s41467-022-30261-3

Nature Communications (May 2022)

A multiplex platform for small RNA sequencing elucidates multifaceted tRNA stress response and translational regulation

Christopher P. Watkins,
Wen Zhang,
Adam C. Wylder,
Christopher D. Katanski,
Tao Pan

Affiliations

Christopher P. Watkins: Department of Biochemistry and Molecular Biology
Wen Zhang: Department of Chemistry, University of Chicago
Adam C. Wylder: Department of Molecular Genetics and Cell Biology, University of Chicago
Christopher D. Katanski: Department of Biochemistry and Molecular Biology
Tao Pan: Department of Biochemistry and Molecular Biology

DOI: https://doi.org/10.1038/s41467-022-30261-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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This paper develops a multiplex RNA-seq method that reports tRNA abundance, modification, charging, and fragmentation. Results show stress-induced regulation in translational elongation and association of modification and tRNA fragment biogenesis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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