Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense
Dongxin Liu,
Wencong He,
Mingxia Jiang,
Bing Zhao,
Xichao Ou,
Chunfa Liu,
Hui Xia,
Yang Zhou,
Shengfen Wang,
Yuanyuan Song,
Yang Zheng,
Qian Chen,
Jiale Fan,
Guangxue He,
Yanlin Zhao
Affiliations
Dongxin Liu
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
Wencong He
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
Mingxia Jiang
Institute for Communicable Disease Control and Prevention, Qinghai Provincial Center for Disease Control and Prevention
Bing Zhao
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Xichao Ou
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Chunfa Liu
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Hui Xia
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Yang Zhou
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Shengfen Wang
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Yuanyuan Song
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Yang Zheng
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Qian Chen
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Jiale Fan
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Guangxue He
Science and Technology Department, Chinese Center for Disease Control and Prevention
Yanlin Zhao
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention
Abstract Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense, via specific erm(41) gene. The amplification was carried out at 65 °C for only 60 min, and the results could be visualized on a lateral flow strip. Positive results only occurred in M. abscessus and M. massiliense, no cross-reaction with other mycobacterial species was observed. Therefore, the cost-effective MABC (M. abscessus complex)–LAMP–LFD method developed here was able to correct the diagnose of M. abscessus and M. massiliense infection in a short time. Thus, this method could be used to guide clinicians in treatment of M. abscessus group infections.
