PLATELET-RICH PLASMA AND FIBROBLASTS: HORMESIS EFFECTS

Abstract

Aim of the study The goal of our study was represented by the analysis of the effects of different concentrations of platelet-rich plasma extracts on the degree of senescence of dermal fibroblasts (by evaluating β-galactosidase) in the presence of metformin and rapamycin. Material and methods Rat dermal fibroblasts from storage were cultivated using conventional laboratory protocols and subsequently assessed for β-galactosidase expression. Then, we used PRP gel in a concentration of 1% and 5%, administered in the culture medium of fibroblasts for 5 cell passages. Moreover, the pharmacologic active substances we used for the various experiments and protocols were metformin (1 µM) and rapamycin (1 µM). Results The results obtained demonstrate that PRP 1% has the effect of transforming fibroblasts into younger cells, especially in the presence of metformin, a programmer of energy metabolism, and then rapamycin, an autophagy stimulator and mTOR pathway activator. It should be noted that a concentration of 5% PRP in the culture medium of fibroblasts for 5 passages has the opposite effect, of their biochemical aging. Conclusions The results obtained demonstrate that PRP 1% has the effect of transforming fibroblasts into younger cells, especially in the presence of metformin and then rapamycin. Moreover, the hormetic effects were demonstrated in the case of platelet-rich plasma concerning cultured fibroblasts.

Keywords

• platelet-rich plasma
• fibroblast
• hormesis
• β-galactosidase
• metformin
• rapamycin