Jichu yixue yu linchuang (Jan 2021)
Inflammatory cytokines and apoptosis related proteins participate in the formation of deep vein thrombosis of lower extremity in rats
Abstract
Objective To investigate the expression and regulation mechanism of IL-1β, TNF-α, Bcl-2 and BAX in rats with inferior vena cava thrombosis. Methods The rats were randomly divided into control group, inferior vena cava thrombus model group, mir-5189-3p inhibitor group and miR-5189-3p inhibitor NC group. Twenty-four hours later, the animals were killed by cervical vertebrae dislocation, blood samples were taken and tissues were collected; the level of IL-1β and TNF-α in blood samples were detected by ELISA; The morphology of inferior vena cava thrombus was microscopied with HE staining; The expression of Bcl-2 and BAX protein in inferior vena cava was detected by Western blot. Results The level of IL-1β and TNF-α in the model group was significantly higher than those in the control group (P＜0.05); the level of miR-5189-3p inhibitor group was significantly higher than that in the control group (P＜0.05); the level of miR-5189-3p inhibitor group was significantly higher than that in the model group (P＜0.05); the level of miR-5189-3p inhibitor negative control group was significantly lower than that in the model group (P＜0.05). In the model group and miR-5189-3p inhibitor group, red thrombus and mixed thrombus were found; the vessel showed inflammatory cell infiltration, and thrombus in the negative control group of miR-5189-3p inhibitor was obviously organized, with signs of blood flow recanalization. Bcl-2/BAX protein level in the model group was significantly lower than that in the control group (P＜0.05); miR-5189-3p inhibitor group was significantly lower than that in the model group (P＜0.05); while miR-5189-3p inhibitor in negative control group was significantly higher than that in the model group (P＜0.05). Conclusions miR-5189-3p can affect the formation of DVT by regulating the expression of inflammatory cytokines and apoptosis related proteins, which provides a new idea and basis for later research.
