Formulation and evaluation of atovaquone-loaded macrophage-derived exosomes against Toxoplasma gondii: in vitro and in vivo assessment

Fatemeh Goudarzi; Vahid Jajarmi; Saeedeh Shojaee; Mehdi Mohebali; Hossein Keshavarz

doi:10.1128/spectrum.03080-23

Microbiology Spectrum (Jan 2024)

Formulation and evaluation of atovaquone-loaded macrophage-derived exosomes against Toxoplasma gondii: in vitro and in vivo assessment

Fatemeh Goudarzi,
Vahid Jajarmi,
Saeedeh Shojaee,
Mehdi Mohebali,
Hossein Keshavarz

Affiliations

Fatemeh Goudarzi: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran
Vahid Jajarmi: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran
Saeedeh Shojaee: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran
Mehdi Mohebali: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran
Hossein Keshavarz: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran

DOI: https://doi.org/10.1128/spectrum.03080-23
Journal volume & issue: Vol. 12, no. 1

Abstract

Read online

ABSTRACT This study aimed to prepare and assess atovaquone (ATQ)-loaded exosomes (EXO-ATQ) against Toxoplasma gondii to improve their therapeutic activities on acute and chronic phases of murine toxoplasmosis. Exosomes were isolated from a mouse macrophage cell line (J774A.1). The isolated exosomes were loaded with ATQ (EXO-ATQ), applying the co-incubation method. In vitro efficacy of EXO-ATQ against T. gondii infection was assessed in Vero cell culture. An in vivo experiment was carried out in BALB/c mice infected with RH (acute strain) and Tehran (chronic strain) of T. gondii, followed by treatment with EXO-ATQ. Their survival time and parasite load were compared with that in the suspension of ATQ (S-ATQ) as a positive control group. The in vitro study showed that exosomes improved the efficacy of ATQ, and EXO-ATQ significantly reduced intracellular proliferation of T. gondii tachyzoites compared to S-ATQ (P ≤ 0.05). In acute experimental toxoplasmosis, the mice treated with EXO-ATQ showed a significant reduction in tachyzoites count of the peritoneal cavity and a longer survival time (P ≤ 0.05). Furthermore, EXO-ATQ administration significantly decreased the mean number (97.3% cyst reduction) and the average size of the brain cysts of chronically infected mice with T. gondii, Tehran strain (P ≤ 0.05). Besides, the decrease in cyst numbers was confirmed by the down-regulation of BAG1 using a real-time PCR assay. Our results implied that exosomes have the potential to be used as an efficient drug delivery system, and the loading of ATQ into the exosomes ameliorates the effect of ATQ against T. gondii. This study suggests a new strategy for improving the effectiveness of ATQ against acute and chronic phases of T. gondii. IMPORTANCE This study is the first of its kind that suggests exosomes as a nano-carrier loaded with atovaquone (ATQ), which could be considered as a new strategy for improving the effectiveness of ATQ against acute and chronic phases of Toxoplasma gondii.

Published in Microbiology Spectrum

ISSN: 2165-0497 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/spectrum

About the journal

Abstract

Keywords