Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

Tatiana Flisikowska; Monika Stachowiak; Hongen Xu; Alexandra Wagner; Alejandra Hernandez-Caceres; Christine Wurmser; Carolin Perleberg; Hubert Pausch; Anna Perkowska; Konrad Fischer; Dmitrij Frishman; Ruedi Fries; Marek Switonski; Alexander Kind; Dieter Saur; Angelika Schnieke; Krzysztof Flisikowski

doi:10.1038/s41598-017-06741-8

Scientific Reports (Jul 2017)

Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

Tatiana Flisikowska,
Monika Stachowiak,
Hongen Xu,
Alexandra Wagner,
Alejandra Hernandez-Caceres,
Christine Wurmser,
Carolin Perleberg,
Hubert Pausch,
Anna Perkowska,
Konrad Fischer,
Dmitrij Frishman,
Ruedi Fries,
Marek Switonski,
Alexander Kind,
Dieter Saur,
Angelika Schnieke,
Krzysztof Flisikowski

Affiliations

Tatiana Flisikowska: Chair of Livestock Biotechnology, Technische Universität München
Monika Stachowiak: Department of Genetics and Animal Breeding, Poznan University of Life Sciences
Hongen Xu: Department of Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München
Alexandra Wagner: Chair of Livestock Biotechnology, Technische Universität München
Alejandra Hernandez-Caceres: Chair of Livestock Biotechnology, Technische Universität München
Christine Wurmser: Chair of Animal Breeding, Technische Universität München
Carolin Perleberg: Chair of Livestock Biotechnology, Technische Universität München
Hubert Pausch: Chair of Animal Breeding, Technische Universität München
Anna Perkowska: Department of Genetics and Animal Breeding, Poznan University of Life Sciences
Konrad Fischer: Chair of Livestock Biotechnology, Technische Universität München
Dmitrij Frishman: Department of Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München
Ruedi Fries: Chair of Animal Breeding, Technische Universität München
Marek Switonski: Department of Genetics and Animal Breeding, Poznan University of Life Sciences
Alexander Kind: Chair of Livestock Biotechnology, Technische Universität München
Dieter Saur: Klinikum Rechts der Isar II, Technische Universität München
Angelika Schnieke: Chair of Livestock Biotechnology, Technische Universität München
Krzysztof Flisikowski: Chair of Livestock Biotechnology, Technische Universität München

DOI: https://doi.org/10.1038/s41598-017-06741-8
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 9

Abstract

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Abstract We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC 1311 truncating mutation orthologous to human APC 1309 , analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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