Separate Effects of Sodium on Germination in Saline–Sodic and Alkaline Forms at Different Concentrations

Abstract

Salinity negatively impacts crop productivity, yet neutral and alkali salt stresses are not often differentiated. To investigate these abiotic stresses separately, saline and alkaline solutions with identical concentrations of sodium (12 mM, 24 mM and 49 mM) were used to compare the seed germination, viability and biomass of four crop species. Commercial buffers containing NaOH were diluted to generate alkaline solutions. The sodic solutions tested contained the neutral salt NaCl. Romaine lettuce, tomato, beet, and radish were seeded and grown hydroponically for 14 days. A rapid germination was observed for alkaline solutions when compared to saline–sodic solutions. The highest plant viability recorded (90.0%) was for the alkaline solution, containing 12 mM Na+, and for the control treatment. Plant viability, with a value of 49 mM Na+ in saline–sodic and alkaline solutions, was the lowest (50.0% and 40.8% respectively), and tomato plants did not germinate. EC values were higher for the saline–sodic solutions than the alkaline solutions, yielding greater fresh mass per plant for all species, with the exception of beets grown in alkaline solution, with a value of 24 mM Na+. The fresh mass of romaine lettuce grown in the 24 mM Na+ saline–sodic solution was significantly greater than romaine lettuce grown in the alkaline solution with the same sodium concentration.

Keywords

• germination
• alkalinity
• sodicity
• salt stress
• hydroponic experiment