Communications Biology (Apr 2021)

Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals

  • Leire Egia-Mendikute,
  • Alexandre Bosch,
  • Endika Prieto-Fernández,
  • So Young Lee,
  • Borja Jiménez-Lasheras,
  • Ana García del Río,
  • Asier Antoñana-Vildosola,
  • Chiara Bruzzone,
  • Maider Bizkarguenaga,
  • Nieves Embade,
  • Rubén Gil-Redondo,
  • María Luz Martínez-Chantar,
  • Marcos López-Hoyos,
  • Nicola G. A. Abrescia,
  • José M. Mato,
  • Óscar Millet,
  • Asís Palazón

DOI
https://doi.org/10.1038/s42003-021-02011-6
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 10

Abstract

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Egia-Mendikute et al. develop a multiplexed flow cytometric bead array to simultaneously detect antibodies reactive to three immunogenic SARS-CoV-2 proteins. More sensitive than ELISA, they detected N-reactive antibodies in COVID-19-negative individuals with this assay, showing it to have superior potential to detect low antibody titres compared to current gold standard serology methods.