Knockdown Of Long Non-Coding RNA TP73-AS1 Inhibited Cell Proliferation And Metastasis Through Wnt/β-Catenin Pathway In Lung Adenocarcinoma

Abstract

Ying Peng,1,* Yan Xu,1,* Guangming Yang,2 Shiwei Li,3 Zhilian Rui1 1Department of Clinical Laboratory, Liyang People’s Hospital, Liyang 213300, People’s Republic of China; 2Department of Pathology, Liyang People’s Hospital, Liyang 213300, People’s Republic of China; 3Department of Clinical Laboratory, Nanjing Clinical Nuclear Medical Center, Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ying PengDepartment of Clinical Laboratory, Liyang People’s Hospital, Nanjing, People’s Republic of ChinaEmail [email protected]: Various evidences showed that abnormally expressed long non-coding RNAs (lncRNAs) play important roles in the tumorigenesis and progression of malignancies. However, the exact role and regulatory mechanism of lncRNA TP73-AS1 in the pathogenesis and progression of lung adenocarcinoma remain to be further elucidated.Purpose: The aim of this study was to investigate the functional role and underlying mechanism of lncRNA TP73-AS1 in lung adenocarcinoma progression.Methods: RT-PCR assay was employed to detect TP73-AS1 expression in lung adenocarcinoma tissues and cells. The function of TP73-AS1 in lung adenocarcinoma progression was estimated by MTT assay, EdU assay, flow cytometry, Western blot, wound-healing assay and transwell assay.Results: LncRNA TP73-AS1 expression was significantly increased in lung adenocarcinoma tissues and cell lines. Moreover, functional assays revealed that silencing of lncRNA TP73-AS1 could attenuate cell proliferation, migration, invasion and epithelial–mesenchymal transition of lung adenocarcinoma, while enhanced expression of lncRNA TP73-AS1 led to the opposite results. Additionally, lncRNA TP73-AS1 knockdown could facilitate cell apoptosis and overexpression of lncRNA TP73-AS1 inhibited cell apoptosis. In addition, we further determined that lncRNA TP73-AS1 regulated cell metastasis through inducing the activation of Wnt/β-catenin signaling pathway in lung adenocarcinoma.Conclusion: Our results indicated that lncRNA TP73-AS1 may play an oncogenic role in lung adenocarcinoma progression, which provided a promising therapy strategy for the treatment of lung adenocarcinoma.Keywords: lncRNA TP73-AS1, proliferation, apoptosis, EMT, Wnt/β-catenin pathway, lung adenocarcinoma

Keywords

• lncrna tp73-as1
• proliferation
• apoptosis
• emt
• wnt/β-catenin pathway
• lung adenocarcinoma