Effects of Short Time-High Voltage Electric Field Treatment on the Accumulation of γ-Aminobutyric Acid in Germinated Buckwheat

Abstract

The short-time high-voltage electric field treatment was used to increase the GABA content of buckwheat after germination, and the activities of GABA shunt enzymes were measured. The results showed that buckwheat was soaked at 35 ℃ for 3 hours, after treatment with 1.0 KV/cm high-voltage electric field for 30 minutes. The buckwheat were incubated at 25 ℃ and 80% humidity for 24 hours. The content of GABA in the control group was (184.38 ± 0.46) mg/100 g, and the content of GABA in the treated group was (195.49 ± 0.81) mg/100 g, which was higher than that in the control group (P<0.05). Compared with the control group, high voltage field treatment significantly increased the activities of glutamic acid decarboxylase (GAD) (P<0.05), inhibited the activities of succinic semialdehyde dehydrogenase (SSADH) (P<0.05), and increased and then decreased the activities of GABA transaminase (GABA-T). Short-time high-voltage electric field treatment can enrich GABA in germinating sweet buckwheat by affecting the activities of GABA shunt enzymes.

Keywords

• gaba
• buckwheat
• high voltage electric field
• germinate
• gaba shunt