Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
Ana C. Fernandes,
Carla M. Duarte,
Filipe A. Cardoso,
Ricardo Bexiga,
Susana Cardoso,
Paulo P. Freitas
Affiliations
Ana C. Fernandes
INESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal
Carla M. Duarte
INESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal
Filipe A. Cardoso
INESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal
Ricardo Bexiga
CIISA, Veterinary Medicine Faculty, Technical University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisbon, Portugal
Susana Cardoso
INESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal
Paulo P. Freitas
INESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk.
