Strontium and selenium doped bioceramics incorporated polyacrylamide-carboxymethylcellulose hydrogel scaffolds: mimicking key features of bone regeneration

Abstract

Polyacrylamide-carboxymethylcellulose hydrogels lack mechanical strength, uncontrolled rate of degradation, and poor osteogenic properties which can be improved by the incorporation of bioceramics. Strontium and selenium doped bioceramics xSrO.(43-x)CaO.40SiO2.(12-y)P2O5.ySeO3.5MgO (where x = 6 mol%, y = 3 mol%) incorporated polyacrylamide-carboxymethylcellulose hydrogels were synthesized by free-radical polymerization. Above mentioned bioceramics were prepared by the sol-gel route. XRD analysis indicated an increase in crystallinity with the increment of bioceramics content. With the incorporation of bioceramics, the apatite phase formation was observed on 1st day of immersion in simulated body fluid at pH 7.4. Strontium and selenium doped bioceramics impregnated hydrogels exhibited an inhibitory effect on human osteosarcoma MG63 cell line. Moreover, bioceramics incorporated hydrogels provide a conducive environment for MC3T3-E1 osteoblast cell line proliferation, adhesion and also exhibited good alkaline phosphatase activity. Synthesized hydrogels indicated special osteogenic properties and can be potentially utilized in the hard tissue regeneration as well as for recovery of bone cancer patients.

Keywords

• bioceramics
• bone tissue regeneration
• carboxymethylcellulose
• hydrogels
• polyacrylamide