Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum Extract
Kangping Wu,
Yilin Fang,
Biyuan Hong,
Yihui Cai,
Honglei Xie,
Yunpu Wang,
Xian Cui,
Zhigang Yu,
Yuhuan Liu,
Roger Ruan,
Qi Zhang
Affiliations
Kangping Wu
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, China
Yilin Fang
Queen Mary School, Jiangxi Medical College, Nanchang University, Nanchang 330047, China
Biyuan Hong
China Coal Zhejiang Testing Technology Co., Ltd., Hangzhou 310000, China
Yihui Cai
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, China
Honglei Xie
Harbin Center for Disease Control and Prevention, Harbin 150000, China
Yunpu Wang
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, China
Xian Cui
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, China
Zhigang Yu
Australian Centre for Water and Environmental Biotechnology (Formerly AWMC), The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia
Yuhuan Liu
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, China
Roger Ruan
Center for Biorefining, Department of Bioproducts and Biosystems Engineering and Department of Food Science and Nutrition, University of Minnesota, Saint Paul, MN 55108, USA
Qi Zhang
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, China
High-cost carbon sources are not economical or sustainable for the heterotrophic culture of Chlorella vulgaris. In order to reduce the cost, this study used sweet sorghum extract (SE) and its enzymatic hydrolysate (HSE) as alternative carbon sources for the heterotrophic culture of Chlorella vulgaris. Under the premise of the same total carbon concentration, the value-added product production performance of Chlorella vulgaris cultured in HSE (supplemented with nitrogen sources and minerals) was much better than that in the glucose medium. The conversion rate of the total organic carbon and the utilization rate of the total nitrogen were both improved in the HSE system. The biomass production and productivity using HSE reached 2.51 g/L and 0.42 g/L/d, respectively. The production of proteins and lipids using HSE reached 1.17 and 0.35 g/L, respectively, and the production of chlorophyll-a, carotenoid, and lutein using HSE reached 30.42, 10.99, and 0.88 mg/L, respectively. The medium cost using HSE decreased by 69.61% compared to glucose. This study proves the feasibility and practicability of using HSE as a carbon source for the low-cost heterotrophic culture of Chlorella vulgaris.
