Frontiers in Cellular and Infection Microbiology (Jan 2020)

Duplicate US1 Genes of Duck Enteritis Virus Encode a Non-essential Immediate Early Protein Localized to the Nucleus

  • Yangguang Li,
  • Yangguang Li,
  • Yangguang Li,
  • Ying Wu,
  • Ying Wu,
  • Ying Wu,
  • Mingshu Wang,
  • Mingshu Wang,
  • Mingshu Wang,
  • YunChao Ma,
  • YunChao Ma,
  • YunChao Ma,
  • Renyong Jia,
  • Renyong Jia,
  • Renyong Jia,
  • Shun Chen,
  • Shun Chen,
  • Shun Chen,
  • Dekang Zhu,
  • Dekang Zhu,
  • Mafeng Liu,
  • Mafeng Liu,
  • Mafeng Liu,
  • Qiao Yang,
  • Qiao Yang,
  • Qiao Yang,
  • Xinxin Zhao,
  • Xinxin Zhao,
  • Xinxin Zhao,
  • Shaqiu Zhang,
  • Shaqiu Zhang,
  • Shaqiu Zhang,
  • Juan Huang,
  • Juan Huang,
  • Juan Huang,
  • Xumin Ou,
  • Xumin Ou,
  • Xumin Ou,
  • Sai Mao,
  • Sai Mao,
  • Sai Mao,
  • Ling Zhang,
  • Ling Zhang,
  • Ling Zhang,
  • Yunya Liu,
  • Yunya Liu,
  • Yunya Liu,
  • Yanling Yu,
  • Yanling Yu,
  • Yanling Yu,
  • Leichang Pan,
  • Leichang Pan,
  • Bin Tian,
  • Bin Tian,
  • Mujeeb Ur Rehman,
  • Mujeeb Ur Rehman,
  • Xiaoyue Chen,
  • Xiaoyue Chen,
  • Anchun Cheng,
  • Anchun Cheng,
  • Anchun Cheng

DOI
https://doi.org/10.3389/fcimb.2019.00463
Journal volume & issue
Vol. 9

Abstract

Read online

The duplicate US1 genes of duck enteritis virus (DEV) encode a protein with a conserved Herpes_IE68 domain, which was found to be closely related to the herpes virus immediate early regulatory protein family and is highly conserved among counterparts encoded by Herpes_IE68 genes. Previous studies found the homologous proteins HSV-1 ICP22 and VZV ORF63/ORF70 to be critical for virus transcription and replication. However, little is known about the DEV ICP22 protein. In this paper, we describe the characteristics of this protein based on pharmacological experiments, real-time quantitative Polymerase Chain Reaction, Western blot, and immunofluorescence assays. We also investigate the role of the protein in DEV replication via mutation of US1. As a result, we found that the DEV ICP22 protein is a non-essential immediate early protein predominantly located in the nucleus of infected DEF cells and that DEV replication is impaired by US1 deletion. We also found that ICP22 contains a classical nuclear localization signal (NLS) at 305-312AA, and ICP22 cannot enter the nucleus by itself after mutating residue 309.

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