Frontiers in Veterinary Science (May 2022)

Involvement of the Actinobacillus pleuropneumoniae ompW Gene in Confrontation of Environmental Pressure

  • Xiabing Chen,
  • Zhiyong Shao,
  • Lijun Wu,
  • Bin He,
  • Wenhai Yang,
  • Jie Chen,
  • Erguang Jin,
  • Qi Huang,
  • Qi Huang,
  • Liancheng Lei,
  • Jiajia Xu,
  • Jiajia Xu,
  • Haotian Li,
  • Haotian Li,
  • Hui Zhang,
  • Hui Zhang,
  • Yun Wan,
  • Wu Liu,
  • Rui Zhou,
  • Rui Zhou

DOI
https://doi.org/10.3389/fvets.2022.846322
Journal volume & issue
Vol. 9

Abstract

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Actinobacillus pleuropneumoniae causes porcine pleuropneumonia. The function of the outer membrane protein W gene (ompW) of A. pleuropneumoniae has not been evaluated. Thus a deletion mutant of ompW, ΔompW, was constructed to explore the effect of ompW gene deletion on bacterial growth, biofilm formation, bacterial morphology, oxidative tolerance, susceptibility to antibiotics, and the expression of ribosome synthesis and ABC transporter related genes. Results showed that the ompW gene deletion did not affect biofilm formation and the growth of A. pleuropneumoniae but did affect bacterial morphology during steady growth, oxidative tolerance, and bacterial susceptibility to polymyxin B, kanamycin, and penicillin. The ompW gene deletion also affected the expression of ribosome synthesis and ABC transporter related genes. These results suggested that ompW may regulate the biological phenotype of A. pleuropneumoniae.

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