Frontiers in Molecular Biosciences (Nov 2021)

Bioinformatic Analyses and Experimental Verification Reveal that High FSTL3 Expression Promotes EMT via Fibronectin-1/α5β1 Interaction in Colorectal Cancer

  • Yuanjie Liu,
  • Yuanjie Liu,
  • Jiepin Li,
  • Jiepin Li,
  • Jiepin Li,
  • Shuhong Zeng,
  • Shuhong Zeng,
  • Ying Zhang,
  • Ying Zhang,
  • Yonghua Zhang,
  • Zhichao Jin,
  • Shenlin Liu,
  • Shenlin Liu,
  • Xi Zou

DOI
https://doi.org/10.3389/fmolb.2021.762924
Journal volume & issue
Vol. 8

Abstract

Read online

Background: Colorectal cancer (CRC) is a typical cancer prevalent worldwide. Despite the conventional treatments, CRC has a poor prognosis due to relapse and metastasis. Moreover, there is a dearth of sensitive biomarkers for predicting prognosis in CRC.Methods: This study used a bioinformatics approach combining validation experiments to examine the value of follistatin-like 3 (FSTL3) as a prognostic predictor and therapeutic target in CRC.Results:FSTL3 was remarkably upregulated in the CRC samples. FSTL3 overexpression was significantly associated with a poor prognosis. FSTL3 was found to activate the epithelial-mesenchymal transition by promoting the binding of FN1 to α5β1. FSTL3 expression was also positively correlated with the abundance of the potent immunosuppressors, M2 macrophages.Conclusion:FSTL3 overexpression affects CRC prognosis and thus, FSTL3 can be a prognostic biomarker and therapeutic target with potential applications in CRC.

Keywords