Population level of main microbiota and yeast-like Candida fungi in women with vulvovaginal candidiasis

Abstract

Aim - to determine population levels of main microbiota and yeast-like fungi of the genus Candida in healthy women and in women with vulvovaginal candidiasis. Materials and methods. For 10 years we have been surveyed 270 women in reproductive age from 19 to 34 years old with vulvovaginal candidiasis and 73 healthy women. In all patients population level of main microflora and yeast-like fungi of the genus Candida in vulvovaginal content has been determined. The isolation of yeast-like fungi of the genus Candida has been performed by seeding of vulvovaginal content on a solid Saboaraud nutrient medium and on Saboaraud medium with the addition of cycloheximide, relatively with pure culture. "Seedlings test" and identification by species with establishment of population level had been performed in each individual case. Results. Coexistence of representatives of the ecosystem "microorganism - microbiota" and compared the changes of microecology of vulvovaginal content in healthy women and women with vulvovaginal candidiasis were defined. In the study of population level of yeast-like fungi of the genus Candida in vulvovaginal content in women with vulvovaginal candidiasis it was found that the dominant species was C. albicans, of population level of which were different from other species, such as C. guillіermondii by 1.77 %, C. krusei - by 4.82 %, C. glabrata - by 5.78 %, C. kefyr – by 8.19 %, C. tropicalis – by 8,35 %, C. inconspicua and C. zeylanoides by 15.08 %. Thus, in the vulvovaginal content of women with vulvovaginal candidiasis yeast-like fungi of the genus Candida of 8 different taxons were at the population level over 5,00 lg CFU/ml, which can not only persist but they were suspected to be agents of inflammation. The results of the study of population levels of autochtonous obligate and facultative bacteria of vulvovaginal content of women with vulvovaginal candidiasis indicated that the level of Lactobacteria decreased by 67.87 %, Bifidobacteria - by 67.27 % compared to the practically healthy women and Propionibacterium were not generally found in minimal (<4.00 lg CFU/ml) quantities. Thus, vulvovaginal candidiasis develops with impaired self-regulating functions of bacteria in the biotope. Conclusions. Definition of indicators of microbiota of vulvovaginal content in women with vulvovaginal candidiasis made it possible to detect high population levels of yeast-like fungi of the genus Candida, such as C. albicans and violation of indigenous self-regulating functions of obligate bacteria that calls for decontamination of Candida and correction of normal flora.

Keywords

• microbiota
• Candida
• vulvovaginal candidiasis