Communications Biology (Jun 2021)

Single-cell RNA sequencing of human nail unit defines RSPO4 onychofibroblasts and SPINK6 nail epithelium

  • Hyun Je Kim,
  • Joon Ho Shim,
  • Ji-Hye Park,
  • Hyun Tae Shin,
  • Jong Sup Shim,
  • Kee-Taek Jang,
  • Woong-Yang Park,
  • Kyung-Hoon Lee,
  • Eun Ji Kwon,
  • Hyung-Suk Jang,
  • Hanseul Yang,
  • Jong Hee Lee,
  • Jun-Mo Yang,
  • Dongyoun Lee

DOI
https://doi.org/10.1038/s42003-021-02223-w
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 13

Abstract

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Kim et al. conducted single-cell RNA sequencing to determine the transcriptome profiles of human nail units using polydactyly specimens to demonstrate mesenchymal and epithelial cell populations, characterized by RSPO4 and SPINK6 respectively. RSPO4 + onychofibroblasts localized with LGR6 + nail matrix, leading to WNT/ β-catenin activation and suggesting a role for onychofibroblasts in onychomatricoma pathogenesis.