Stem Cell Research (Dec 2022)

Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

  • Dasom Mun,
  • Ji-Young Kang,
  • Yumin Chun,
  • Da-Seul Park,
  • Hyoeun Kim,
  • Nuri Yun,
  • Seung-Hyun Lee,
  • Boyoung Joung

Journal volume & issue
Vol. 65
p. 102940

Abstract

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PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and spontaneous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.